报告人👩🏻⚕️：Dr. William J. Kelly，Professor of Chemical Engineering，Villanova University, USA
Dr. William J. Kelly
Professor of Chemical Engineering
Villanova University, USA
Dr. William J. Kelly is Professor of chemical engineering at Villanova University in Philadelphia, Pa, USA. Dr. Kelly has over 25 years of experience with bioprocesses as a bioprocess engineer at Merck and Company and as a researcher and teacher at Villanova. He obtained at Ph.D. from Penn State University under the direction of Dr. Art Humphrey.
While at Merck, Dr. Kelly worked on bioprocesses ranging from 1 – 50,000 liters in scale. His primary function was to improve the performance of fermentations responsible for producing the active ingredient in some of Merck’s antibiotics and vaccines. This work included optimization of media composition and sterilization, as well as oxygen transfer and mixing. Dr. Kelly was involved in the operation and optimization of large scale bio-processing equipment including centrifuges, filtration and chromatography skids.
A Professor at Villanova since 1999, Dr. Kelly has established several new undergraduate and graduate level biochemical engineering courses, and is currently director of Villanova’s MS. Program in Biochemical Engineering. During this seminar, Dr. Kelly will speak about graduate studies in Engineering at Villanova. Dr. Kelly has an active research program in upstream and downstream biopharmaceutical processing, and will also talk about a recent project in which he collaborated with Janssen Inc. For this project, an experimental setup was devised that allowed for determination of the time it took for membrane fouling to occur for given mammalian (PER.C6) cell culture cell densities and viabilities as permeate flowrate and antifoam concentration was varied. The experimental results indicate, in accordance with D’Arcy’s law, that the average resistance to permeate flow (across a cycle of operation) increases as biological material deposits on the membrane. Scanning Electron Microscope (SEM) images of the post-run filtration surface indicated that both cells and antifoam micelles deposit on the membrane. A unique mathematical model, based on the assumption that fouling was due to pore blockage from the cells and micelles in combination, was devised that that allowed for estimation of sticking factors for the cells and the micelles on the membrane. This model was then used to accurately predict the increase in transmembane pressure (TMP) during constant flux operation for an ATF cartridge used for perfusion cell culture.
a. Professional Preparation.
Institution Major Degree Year
Clarkson University, Potsdam, NY Chem. Eng. B.S. 1985
University of Virginia, Charlottesville, VA Chem. Eng M.S. 1988
Penn State University, State College, PA Chem. Eng. Ph.D. 1998
2015-present Professor, Department of Chemical Engineering, Villanova University, Villanova, PA
2005-2015 Associate Professor, Department of Chemical Eng., Villanova University, Villanova, PA
1999-2005 Assistant Professor, Department of Chemical Eng., Villanova University, Villanova, PA
1989-1999 Biochemical Engineer, Merck and Company, West Point, PA
[1] Kelly, W, Scully, J, Zhang, D, Feng, G.Lavengood, M., Condon, J, Knighton, J, Bhatia, R.
Understanding and Modeling Alternating Tangential Flow Filtration for Perfusion Cell Culture. Biotechnology Progress. 2014;30(6): 1291-1300
[2] Kamaraju, V, Wetzel, K, Kelly, W. Modeling Shear Induced CHO Cell Damage in a Rotary Positive
Displacement Pump. Biotechnology Progress. 2010;26(16):1610-161.
[3] Kelly, W. A Review: Using CFD to characterize and Improve Bioreactor Performance. Biotechnol.
Appl. Biochem.2008;49:225-231.
[4] Vickroy, B, Lorenz, K, Kelly, W. Modeling shear damage to suspended CHO cells during cross-flow
filtration. Biotechnology Progress. 2007; 23: 194-199
OTHER SIGNIFICANT PRODUCTS
[1] Balsamo, R, .Kelly, W, Satrio, J, Ruiz-Felix, N,.Fetterman, M, Wynn, R, Hagel, K.
Utilization of grasses for potential biofuel production and phytoremediation of heavy metal
contaminated soils. International Journal of Phytoremediation. 2014;17(5):448-455
[2] Kelly, W, Garcia, P, McDermott, S, Kamguia, G, Mullen, P, Jones, G, Ubiera, A, Göklen, K.
Experimental characterization of next generation expanded bed adsorbents for capture of a
recombinant protein expressed in high cell density yeast fermentation. Biotechnology and
Applied Biochemistry. 2013;60(5): 510-520.
[3] Kelly, W, Ubiera, A, Kamguia, G, Mullen, P, GÖklen, K, Huang, Z, Jones, G. Using a two species
competitive binding model to predict expanded bed breakthrough of a recombinant protein
expressed in a high cell density fermentation. Biotechnology and BioProcess Engineering.
[4] Kelly,W,Scully, J, Rubin,J, Kamaraju, V, Kioworski, P, Bhatia,R. Understanding and modeling
Retention of Mammalian Cells in Fluidized Bed Centrifuges. Sumitted to Biotechnology.
[5] Kelly, W, Balguri, M, Asoor, A, Piccini, J, Muske, K. Evaluation of Optimization Metrics for
Continuous Fermentation of PlasmidDNA.,Chemical Engineering & Technology. 2009;
d. Synergistic activities.
1. Classroom innovation: Participated in a week-long workshop on “Entrepreneurship in Engineering Education: and developed a two-day module on “Genomics” for the Fall 2015 “Introduction to Biotechnology (CHE5532)” course. Also, was co-author on the ASEE paper that won best CHE paper (i.e. the Martin award) at the 2015 ASEE conference.
2. Board member for two Professional Organizations: Invited member of ESBES (European Society of Biochemical Engineering): 2010 - 2015. Three times elected asmember of ACS-BIOT (American Chemical Society-Biotechnology Division) Executive Board (as “Alternate Councilor”): 2007-2016.
3. Organized a group of twelve US academic and industrial bioscientiststo represent ACS-BIOT at the ESBES meeting in Nice, France in Fall 2015. Was lead PI for this NSF-funded activity. While in Nice, led a “Future Planning Meeting” which has led to plans for a EU—US student bioprocess design competition that will finalize in 2017.
4. Creator and faculty advisor for newly (2015) formed Mid-Atlantic BIOT graduate student chapter. Formed ACS-BIOT’s first (graduate) student chapter. Worked with Faculty at UPenn and U. Delaware to identify and eventually appoint student officers for the chapter from Villanova, UPenn and U. Delaware.
5. Academic and Research advising: I am currently serving as academic advisor for 32 Junior chemical engineering students. In addition, I am currently mentoring 3 undergraduates, 1 M.S., and 1 PhD student in research projects that range from viral recovery using membrane chromatography to lipopeptide purification to optimization of cell culture perfusion processes in microbioreactors.
e. Collaborators & other affiliations.
1.Collaborators.
Dr. Ravi Bhaia Janssen BioPharmaceuticals
Dr. Kent Goklen Glaxo SmithKline Pharmaceuticals
Dr. Kim Clarke Stellenbosch University, South Africa
Ph.D. Advisor= Dr. Arthur Humphrey, Retired
I am currently the thesis advisor for one Ph.D student (Jamie Crawford) at Villanova University.
